BSc (Sydney) Biochemistry, PhD (Australian National University) Biophysical Chemistry
Position: 
Professor
Room: 
331
Phone: 
+61 7 336 54180
Biography: 

After my PhD I held postdoctoral positions at the RSC (ANU) (1987-1988) and at the University of Groningen (1989-1990). In 1990 I moved with W.F. van Gunsteren to ETH Zurich becoming Oberassistant in 1996. In 1998 I was appointed Professor of Biophysical Chemistry, University of Groningen. In 1998 I was awarded the Swiss Ruzicka Prize for research in Chemistry. In 2004 I was awarded an Australian Research Council (ARC) Federation Fellowship and joined The University of Queensland in 2005. In 2011 I was awarded a University of Queensland Vice Chancellor's Senior Research Fellow. I am also an affiliate of the Institute of Molecular Biosciences at UQ and the Australian Infectious Diseases Research Centre.

Brief Research Description: 
Physical and Computational Chemistry - Simulation of biomolecular systems
Research Focus and Collaborations: 


The group focuses on understanding and predicting the macroscopic (experimentally observable) properties of biomolecular systems such as proteins, nucleic acids and lipid aggregates, in terms of the interactions between atoms. In particular our work concentrates on the development of tools (i.e. simulation software, atomic force fields, theoretical models and experimental techniques) that can be used to understand and predict the physico-chemical basis of interactions and dynamic processes within biomolecular systems. Specific areas of interest include structure prediction, protein and peptide folding, the self-assembly of protein/lipid complexes and the calculation of thermodynamic properties such as ligand binding affinities.

Protein Folding
Understanding how proteins fold is one of the grand challenges of modern biology. The failure of proteins to fold correctly is also linked to a range of debilitative diseases including Alzheimer’s disease, BSE and some forms of Type II diabetes where misfolded proteins form destructive aggregates called amyloid fibrils. Research on folding is conducted at multiple levels. Small model systems such as antimicrobial peptides are used to refine force fields and simulation techniques. On a larger scale we are simulating how multiple copies of certain peptides aggregate in order to understand how amyloid fibrils form.

Cell Surface Receptors
How the binding of a molecule to an extracellular receptor transfers a signal across the cell membrane or how changes in the environment can activate certain cell surface receptors are both critical question in cell biology. To address such issues we are investigating the mechanism by which low pH triggers the activation of the Dengue E protein, which plays a critical role in the entry of the virus into cells. We are also investigating the structural changes associated with the binding of human growth hormone to the growth hormone receptor.

Membrane Protein Assembly
Cell membranes are the archetypal self-organised supramolecular structure. Membrane protein complexes also represent a new frontier in structural biology. Using simulations, we are able to directly investigate how bilayers and vesicles form. We are also investigating the assembly of functional structures such as the assembly of anti-microbial peptides into transmembrane pores. This in turn is being used to understand the mechanism by which larger complexes form in heterogeneous environments.

Computational Drug Design
Work in computational drug design is focused in two areas. The development of an automated topology build to provide atomic descriptions of drug like molecules, the development of novel methods for estimating the free energy of binding and for the refinement of non-standard ligand molecules in X-ray crystal complexes.

Research Projects

  • Simulating peptide folding and assembly
  • Pore-forming peptides as models for protein assembly
  • The nucleation and growth of amyloid fibrils
  • Mechanism of activation of the human growth hormone receptor
  • New methods in drug design
Funded Projects: 

NHMRC Project 2015-2018
A New Paradigm for Class I Cytokine Receptor Activation.
Andrew Brooks, Michael J. Waters, Alan Mark
Total value of grant: $919,835

ARC Discovery 2015-2017
Force Fields for Structure Refinement and Computational Drug Design.
Alan Mark, Daan Geerke, Gunnar Klau
Total value of grant: $354,000

ARC Discovery 2013-2015
Membrane proteins: Understanding biological switches, motors and triggers.
A E Mark, M L O'Mara
Total value of grant: $946,000

NHMRC Project 2013-2015
Selective targeting of microbes by peptides of the innate immune system.
A E Mark, F Separovic, M-I Aguilar, D Poger
Total value of grant: $606,000

NHMRC Project 2013-2015
Understanding multidrug resistance in cancer: identification of the substrate and inhibitor binding sites in P-glycoprotein.
M L O'Mara, A E Mark, R Callaghan
Total value of grant: $275,000

NHMRC Project 2012-2014
Structural biology of bacterial lipid IIglycopeptide
antibiotic interactions.
M A Cooper, A E Mark
Total value of grant: $585,000

ARC Discovery 2011-2013
Understanding sub-cellular systems at the atomic level.
A E Mark
Total value of grant: $300,000

NHMRC Project 2011-2013
Development of potent and selective blockers of acid sensing ion channels for the treatment of pain.
G F King, A E Mark, M A Cooper, P Alewood, L Rash
Total value of grant: $558,000

ARC Discovery 2008-2010
From structures to systems: A hierarchical approach to understanding sub-cellular components.
A E Mark, W F van Gunsteren, S-J Marrink
Total value of grant: $398,000

ARC Linkage 2008-2010
Development of cryopreservaton for high value provenance collections of recalcitrant plant species used in post-mining restoration.
R L Mancera, G J Bryant, A E Mark, S R Turner, P Che, E Bunn
Total value of grant: $807,000

Teaching: 
Biophysics Major Convenor  
CHEM1022 Chemistry for the Health Professions
CHEM3011 Advanced Physical Chemistry

 

Significant Professional Activities and Awards: 
1998 Ruzicka Prize for research in Chemistry
2005-2010 Australian Grants Council Federation Fellow.
2005-present Extraordinary Professor “Computer simulation of biological molecules,” University of Groningen, The Netherlands.
2012-present University of Queensland Vice Chancellor's Senior Research Fellow.
2012 The Association of Molecular Modellers of Australasia (AMMA) medal.
2013-2015 Chair of the Merit Allocation Committee for the National Cyber Infrastructure
2013-2015 Australian Grants Council Discovery Outstanding Researcher Award (DORA)

 

International Research Collaborations

The group has a wide range of ongoing collaborations both within Australia and Internationally in Europe and the USA including:

Prof. Wilfred van Gunsteren, ETH, Zurch, Switzerland.

Assoc. Prof Daan Geerke, VU, Amsterdam, The Netherlands.

Prof. Gunar Klau, CWI, Amsterdam, The Netherlands.

Prof. Siewert-Jan Marrink, University of Groningen, The Netherlands.

Dr Jane Allison, Massey University, New Zealand.

Group Members:

Dr David Poger Research Fellow

Selected Publications: