Group leader in protein chemistry and enzymology.
Member of UQ's Latin American Study Group (LASG) - representative for the Faculty of Science.
I obtained a Diploma in Chemistry from the University of Berne in Switzerland in 1992. Upon receiving the award of an International Postgraduate Research Scholarship (IPRS) I joined The University of Queensland (in 1993) to carry out research towards a PhD in Biochemistry (supervisors: Assoc. Profs. Peter Nixon and Ron Duggleby). I graduated in 1997 and continued postdoctoral research at The University of Queensland under the guidance of Profs John de Jersey, Susan Hamilton and Graeme Hanson. During this period I was awarded Fellowships from both the Wellcome Trust and The University of Queensland. In 2000 I spent several months in the Department of Chemistry at the University of Newcastle-upon-Tyne, England, collaborating with Prof. Geoff Sykes, and later that year I commenced a Postdoctoral Fellowship in the Chemistry Department at Stanford University (advisor: Prof. Edward Solomon). In 2003 I was appointed to a Lectureship in (Bio)physical Chemistry at The University of Queensland. In 2006 I was promoted to Senior Lecturer. Following the award of a University of Queensland Foundation Research Excellence Award (in 2007) I was promoted to Associate Professor (in 2009). Since 2011 I also hold a professorial appointment at the National University of Ireland - Maynooth (NUIM). Most recently, in 2012, I have been awarded an ARC Future Fellowship (commences in April 2013).
My research lies at the interface between biochemistry, and biotechnology, inorganic and physical chemistry. Methodologies applied range from protein expression, purification and characterisation, mutagenesis, steady- and pre-steady state enzyme kinetics to various types of molecular spectroscopy. Local collaborators are Profs Luke Guddat, Ross McGeary, Lawrence Gahan, Graeme Hanson, Mark Riley, Paul Meredith.
Specifically, I am interested in the study of the reaction mechanisms of enzymes requiring metal ions for their catalytic function. In particular, my current research focuses on a group of binuclear metallohdrolases, which play crucial roles in a variety of metabolic functions, including bone and collagen resorption, prevention of oxidative damage, correction of errors in DNA replication and cell proliferation. Some of these enzymes¸the metallo-β-lactamases, are also major contributors to the growing emergence of antibiotics-resistant pathogens. My aim is to gain detailed insight into the geometric and electronic structures of these enzymes to develop and design transition state analogues and specific inhibitors of chemotherapeutic and medicinal significance.
I am also interested in enzymes that degrade organophosphate pesticides and nerve agents. These enzymes have gained increasing attention due to their great potential as bioremediators and anti-warfare agents (collaboration with Prof. David Ollis, ANU).
Studies on protein systems are complemented by studies on relevant model complexes (biomimetics; collaboration with Prof. L. Gahan, UQ). In addition to these predominantly mechanistic investigations I also engage in comparative studies to define sequence and structural fingerprints that contribute to metal ion selectivity in protein active sites, and I am interested in evolving enzymatic activities in vitro to perform desired tasks (collaboration with Prof. D. Ollis, ANU). Last but not least, I am also interested in probing the factors that contribute to functional promiscuity in enzyme-catalysed reactions, a phenomenon that has attracted increasing significance since it also harbours the potential to tune enzymes to perform particular tasks that may be beneficial to a variety of applications.
International Research Collaborations:
|Prof. Ademir Neves||Universidade de Santa Catarina, Florianopolis, Brazil|
|Prof. Jim Larrabee||Middlebury College, Middlebury, USA|
|Prof. Alvan Hengge||Utah State University, Logan, USA|
|Prof. Stefano Ciurli||University of Bologna, Bologna, Italy|
|Prof. Peter Comba||University of Heidelberg, Heidelberg, Germany|
|Prof. Adolfo Horn||Universidade Estadual de Norte Fluminense, Fluminense, Brazil|
NHMRC Project Grant 2015-2017
An integrated approach to combat antibiotic resistance
NHMRC Project Grant 2010-2012
Metallo-β-lactamases: predicting and combating antibiotic resistance
Total value of grant: $512,000
ARC Discovery Grant 2012-2014
Organophosphate pesticide degradation: evolved enzymes and biomimetics for bioremediation and medicine
Total value of grant: $285,000
ARC Future Fellowship 2013-2017
Metals in biocatalysis
Total value of grant: $821,000
Bio-physical and bio-inorganic chemistry including chemical and enzyme kinetics, thermodynamics, and chemical equilibrium, as well as bioinorganic chemistry and electron paramagnetic resonance.
PhD Students (15): S. Smith, K. Hadler, E. Leung, F. Ely, P. Vella, A. Kan, L. Daumann, S. Wong, A. Bhumkar, N. Pue, M. M. Pedroso, D. Feder, S.H. Mohd-Pahmi, O. Arjomandi, D. Tan
Honours (11): L. McDonald, P. Herrald, T. Elliott, P. Lee, S. Wong, R. Aston, R. Buchholz, R. Mirams, P. Vella, M. Zee, B. McCarthy, D. Feder, L. Sung